Target Analysis / Targeted Metabolic Profiling

Target Analysis/Classes Profiling
HPLC-API-MS-PDA coupling is perfectly suited for target analysis and classes profiling. High speed RP Chromatography with the use of commercial silica based monolithic C-18 columns offers short time analysis. FINNIGAN LTQ allows to perform qualitative and quantitative analysis in a full scan mode as well as SIM and SRM modes. Online CID fragmentation experiments in both positive and negative modes giving an opportunity for identification/elucidation of unknown detected components as well as verification of the known ones. Parallel UV/VIS data acquisition offers double check for known targets as well as giving valuable information on unknowns and on components that can not be ionized.

Nucleotides separations with LC-ESI-MS-PDA coupling. Standards mixture: A,C,U,G, their MPs,DPs,TPs.	Flavanoids separations with LC-ESI-MS-PDA coupling. Arabidopsis Thalianaleaf acidic water/methanol extract.

Applied Biosystems Qtrap 4000
	• The ability to quantify known small molecules in a complex background matrix. • The ability to monitor up to 300 "targets" within a single chromatography run using MRM. • Targeted Metabolomics: Monitoring small molecules that belong to certain pathways. • Installed and coupled with the Waters UPLC system.

Created by scholz
Vladimir Tolstikov
Last modified 2008-09-19 17:24

Metabolomics Core Lab